The Evolution of Fluorescence Lifetime Imaging: Bridging Diagnostics and Molecular Dynamics Introduction
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Write this down at the top of your calendar. This is your "Feature." This "decay time" is a unique molecular fingerprint [2]
High Sensitivity: It helps surgeons pinpoint where a tumor ends and healthy tissue begins with high accuracy [11]. 7]. Key Advantages in Medicine
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Unlike traditional fluorescence imaging, which measures the intensity of light, FLIm measures the fluorescence lifetime—the exact time a molecule stays in an excited state before emitting light [7]. This "decay time" is a unique molecular fingerprint [2]. Because it is sensitive to the local environment (like pH or viscosity), FLIm can reveal biochemical changes that simple intensity-based images miss [5, 7]. Key Advantages in Medicine